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KMID : 1134820110400081092
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Journal of the Korean Society of Food Science and Nutrition
2011 Volume.40 No. 8 p.1092 ~ p.1098
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Effect of Dietary Supplementation of β-Carotene on Hepatic Antioxidant Enzyme Activities and Glutathione Concentration in Diabetic Rats
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Jang Jung-Hyun
Lee Kyeung-Soon
Seo Jung-Sook
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Abstract
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The present study was conducted to investigate the effect of β-carotene on the antioxidant system of rats with diabetes. Forty Sprague Dawley rats were fed the AIN-76 control diet or the same diet supplemented with β-carotene (7.2 ㎎/㎏ diet) for 3 weeks, then diabetes was induced in half the rats by administering streptozotocin (45 ㎎/㎏ BW) into the femoral muscle. Diabetic and normal rats were fed the experimental diets for 2 more weeks. To investigate the effect of dietary β-carotene on diabetes, the activities of antioxidative enzymes and glutathione concentration were determined in normal and streptozotocin-induced diabetic rats. The plasma glucose levels in diabetic rats were not influenced by the dietary supplementation of β-carotene. Hepatic activities of catalase and superoxide dismutase in diabetic rats were significantly lower than those of control rats but β-carotene tended to induce these activities. Glutathione-S-transferase activity was not significantly different between experimental groups. Glucose-6-phosphatase activity was induced in diabetic rats, but dietary supplementation of β-carotene reduced this activity. The hepatic concentration of reduced glutathione in diabetic rats was lower than that of control rats, but dietary supplementation with β-carotene restored the content to some extent. These data suggest that diabetic rats are exposed to increased oxidative stress and that dietary supplementation with β-carotene may reduce its detrimental effects.
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KEYWORD
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β-carotene, diabetic rat, antioxidative enzyme, glutathione
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